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E-GEOD-24169 - Finding direct target genes of VND7

Status
Released on 31 March 2011, last updated on 19 February 2019
Organism
Arabidopsis thaliana
Samples (20)
Array (1)
Protocols (8)
Description
Direct target genes of VND7 were explored with inducible expression system using glucocorticoid receptor (GR). Transgenic plants expressing 35S:VND7-VP16-GR were treated with dexamethazone (DEX) and/or protein synthesis inhibitor cycloheximide (CHX). A number of genes related to the formation of vascular vessel was induced by DEX even in the presence of CHX. Total RNAs of the transgenic plants expressing 35S:VND7-VP16-GR treated with DEX plus CHX and those treated with CHX only were compared. As a control experiment, transgenic plants harboring empty vector were treated similarly and the total RNAs were compared similarly to identify genes merely induced by DEX treatment itself.
Experiment type
transcription profiling by array 
Contacts
Nobuitaka Mitsuda <nobutaka.mitsuda@aist.go.jp>, Masatoshi Yamaguchi, Misato Ohtani, Nobutaka Mitsuda, Taku Demura
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-24169.idf.txt
Sample and data relationshipE-GEOD-24169.sdrf.txt
Raw data (1)E-GEOD-24169.raw.1.zip
Processed data (1)E-GEOD-24169.processed.1.zip
Array designA-AFFY-2.adf.txt
Links