Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.
E-GEOD-23827 - Contribution of CgPDR1-regulated genes in enhanced virulence of azole-resistant Candida glabrata (part 1)
Released on 31 December 2010, last updated on 2 June 2014
In this study, we aimed to determine genome-wide changes in gene expression driven by seven individual CgPDR1 hyperactive alleles as compared to wild-type allele to identify i) the CgPdr1p target genes differentially expressed in presence of CgPDR1 hyperactive alleles and ii) potential virulence factor(s) regulated by CgPDR1 hyperactive alleles. Microarray experiments revealed a high number of genes (ranging from 80 to 400 genes) differentially regulated by individual CgPDR1 hyperactive alleles. Gene expression was measured in 7 C. glabrata laboratory strains (SFY101, SFY103, SFY105, SFY109, SFY111, SFY115, SFY116) expressing different CgPDR1 hyperactive alleles, in 1 strain (SFY114) expressing a wild-type CgPDR1 allele and in one strain deleted for CgPDR1. The one-color system was used. 3 independent experiments were performed using 3 biological replicates for each strain.
transcription profiling by array
Dominique Sanglard <Dominique.Sanglard@chuv.ch>, Sélène Ferrari
Contribution of CgPDR1-regulated genes in enhanced virulence of azole-resistant Candida glabrata. Ferrari S, Sanguinetti M, Torelli R, Posteraro B, Sanglard D. , PMID:21408004