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E-GEOD-23759 - Differential gene expression profile in skin of Hoxc13 gene-targeted mice

Status
Released on 25 August 2010, last updated on 10 June 2011
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (6)
Description
Hoxc13 has been shown to be essential for proper hair shaft differentiation as Hoxc13 gene-targeted (Hoxc13tm1Mrc) mice completely lack external hair. Because of the phenotypic parallels to the Foxn1nu (nude) mutant mice, we tested whether Hoxc13 and Foxn1 act in a common pathway of hair follicle (HF) differentiation. We show that the alopecia exhibited by both the Hoxc13tm1Mrc and Foxn1nu mice is due to strikingly similar defects in hair shaft differentiation and that both mutants suffer from a severe nail dystrophy. These similarities are consistent with the overlap of Hoxc13 and Foxn1 expression patterns in the HF and nail matrix. DNA microarray analysis of scapular skin from Hoxc13tm1Mrc mutant (mut) compared to wild type (wt) mice identified Foxn1 as significantly down-regulated along with numerous hair keratin genes. This Foxn1 down-regulation apparently reflects the loss of direct transcriptional control by HOXC13 as indicated by our results obtained through co-tranfection and chromatin immunoprecipitation (ChIP) assays. Total number of samples was 6. The experiment was perfomed in triplicate involving 3 indendent wild type skin samples (sample 1, 2, and 3) and 3 independent mutant skin samples (sample 4, 5, and 6).
Experiment type
transcription profiling by array 
Contacts
Christopher S. Potter <christopher.potter@jax.org>, Alan Godwin, Alexander Awgulewitsch, Christopher S Potter, John P Sundberg
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-23759.idf.txt
Sample and data relationshipE-GEOD-23759.sdrf.txt
Raw data (1)E-GEOD-23759.raw.1.zip
Processed data (1)E-GEOD-23759.processed.1.zip
Array designA-GEOD-9746.adf.txt
R ExpressionSetE-GEOD-23759.eSet.r
Links