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E-GEOD-23662 - The effect of sex on gene expression variation within and between populations of Drosophila melanogaster

Status
Released on 23 December 2010, last updated on 2 May 2014
Organism
Drosophila melanogaster
Samples (112)
Array (1)
Protocols (7)
Description
To investigate the effect of sex on within- and between-population variation in gene expression, we performed a microarray analysis of adult females from 16 strains of Drosophila melanogaster, including eight strains from the putative ancestral range in sub-Saharan Africa and eight strains from a European population. The results were compared to those of a previous study of adult male gene expression variation among the same strains (GSE8843). We used dual-channel microarrays to compare genome-wide expression profiles in adult females from 16 inbred strains derived from two natural populations. In total, 56 hybridizations were performed including dye-swaps. The hybridization scheme consisted of a balanced loop design, which allowed an unbiased comparison of relative expression levels within and between populations.
Experiment type
transcription profiling by array 
Contacts
John Parsch, Lena Müller, Rayna Stamboliyska, Sarah S Saminadin-Peter, Stephan Hutter, Wolfgang Stephan
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-23662.idf.txt
Sample and data relationshipE-GEOD-23662.sdrf.txt
Raw data (2)E-GEOD-23662.raw.1.zip, E-GEOD-23662.raw.2.zip
Processed data (1)E-GEOD-23662.processed.1.zip
Array designA-GEOD-3603.adf.txt
R ExpressionSetE-GEOD-23662.eSet.r
Links