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E-GEOD-23635 - Conversion of mouse fibroblast to hepatocyte-like cells by defined factors

Status
Released on 11 May 2011, last updated on 31 May 2011
Organism
Mus musculus
Samples (13)
Array (1)
Protocols (8)
Description
Plasticity of differentiated cells has been proved by nuclear transfer, induced pluripotent cells and transdifferentiation. Here we show that by transduction of 3 factors (Hnf1alpha, Gata4, and Foxa3) and p19Arf inactivation, tail-tip fibroblasts can be converted to hepatocyte-like (iHep) cells, expressing hepatocyte marker genes, and acquiring many mature hepatocyte functions in vitro and in vivo. p19Arf-null TTFs were tranfected with 3 liver enriched transcription factors, then changed to modified Block's medium. To enrich iHep cells, epithelial cells were enriched by partial trypsin digestion.
Experiment type
transcription profiling by array 
Contacts
Lijian Hui, Pengyu Huang
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-23635.idf.txt
Sample and data relationshipE-GEOD-23635.sdrf.txt
Raw data (1)E-GEOD-23635.raw.1.zip
Processed data (1)E-GEOD-23635.processed.1.zip
Array designA-MEXP-724.adf.txt
Links