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E-GEOD-23588 - Influence of Autoinducer-2 (AI-2) in Salmonella Typhimurium Gene Expression

Status
Released on 31 July 2015, last updated on 19 August 2015
Organism
Salmonella enterica subsp. enterica serovar Typhimurium
Samples (26)
Array (1)
Protocols (25)
Description
Salmonella enterica serovar Typhimurium is known to synthesize and respond to the cell signaling molecule, autoinducer-2 (AI-2). The luxS gene is involved in the synthesis of AI-2. We have previously shown that luxS controls a variety of bacterial processes in S. Typhimurium. In this study we identified the genes regulated by AI-2 using mRNA samples from isogenic luxS gene mutant of S. Typhimurium strain 87-26254 grown in LB media in the presence/absence of in vitro synthesized AI-2. In the presence of in vitro synthesized AI-2, 536 genes were significantly (p<0.05) regulated. Interestingly, in vitro synthesized AI-2 caused the down-regulation of 39 genes in Salmonella Pathogenicity Island-1 (SPI-1) including the transcriptional regulators hilA, hilD, and invF. Purified cDNAs from the mutant supplemented with AI-2 and mutant without AI-2 (PBS) were each labeled with Cy-3 mono-Reactive Dye and Cy-5 mono-Reactive Dye (GE Health Care, Piscataway, NJ) and were processed using a dye-swapping design. A total of eight microarray arrays, each with duplicate spots for each gene and used for mutant supplemented with AI-2.
Experiment type
transcription profiling by array 
Contacts
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-23588.idf.txt
Sample and data relationshipE-GEOD-23588.sdrf.txt
Raw data (1)E-GEOD-23588.raw.1.zip
Processed data (1)E-GEOD-23588.processed.1.zip
Array designA-GEOD-10785.adf.txt
Links