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E-GEOD-23528 - Light/dark- and temperature-regulated transcriptional rhythms in adult Caenorhabditis elegans

Status
Released on 31 August 2010, last updated on 2 May 2014
Organism
Caenorhabditis elegans
Samples (95)
Array (1)
Protocols (8)
Description
Most organisms have an endogenous circadian clock that is synchronized to environmental signals such as light and temperature. Although circadian rhythms have been described in the nematode C. elegans at the behavioral level, these rhythms appear to be relatively non-robust. Moreover, in contrast to other animal models, no circadian transcriptional rhythms have been identified. Thus, whether this simple nematode contains a bona fide circadian clock remains an open question. We used microarray experiments to identify light- and temperature-regulated transcriptional rhythms in C. elegans, and show that subsets of these transcripts are regulated in a circadian manner. In addition, we find that light and temperature also globally drive the expression of many genes, indicating that C. elegans exhibits systemic responses to these stimuli. Populations of growth-synchronized wild-type C. elegans L1 larvae were entrained for 5 days until adulthood to 12:12 hr light/dark (LD) cycles (500-1000 lux) at a constant temperature of 18°C, or for 4 days to 12:12 hr temperature cycles (25:15°C - warm/cold or WC) in constant darkness. RNA was collected every 4 hrs during the last entrainment and the subsequent free-running days and analyzed via hybridization of Affymetrix GeneChips. L4 larvae were transferred to FUDR-containing plates to inhibit embryonic development.
Experiment type
transcription profiling by array 
Contacts
Alexander Martinus van der Linden <avanderlinden@unr.edu>, Alexander M van der Linden, Joseph Rodriguez, Matthew Beverly, Michael Rosbash, Piali Sengupta, Sara Wasserman, Sebastian Kadener
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-23528.idf.txt
Sample and data relationshipE-GEOD-23528.sdrf.txt
Raw data (1)E-GEOD-23528.raw.1.zip
Processed data (1)E-GEOD-23528.processed.1.zip
Array designA-AFFY-60.adf.txt
R ExpressionSetE-GEOD-23528.eSet.r
Links