Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.
E-GEOD-22848 - Transcription analysis of AI-2 signal add in experiment in Yersinia pestis CO92 at 37°C
Released on 5 September 2013, last updated on 2 June 2014
Yersinia pestis, the etiological agent of plague, is able to sense cell density by quorum sensing. The function of quorum sensing in Y. pestis is not clear. Here, the process of autoinducer-2 (AI-2) quorum sensing was investigated by comparing transcript profiles when AI-2 quorum-sensing signal is added in. The strain ∆pgm(pigmentation-negative) mutant R88 was used as wild type. The control consisted of cells grown and treated under the same conditions without added signals. Six independent RNA samples from Y. pestis CO92 ∆pgm cultures were paired with six independent RNA samples from AI-2 signal added cultures for hybridization to six two-color microarrays. A dye-swap design was used to remove the Cy5 and Cy3 dye bias.
transcription profiling by array
Chris Minion, Jing Yu
Analysis of Autoinducer-2 Quorum Sensing in Yersinia pestis. Yu J, Madsen ML, Carruthers MD, Phillips GJ, Kavanaugh JS, Boyd JM, Horswill AR, Minion FC. , PMID:23959719