Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-22670 - Ahringer AB9050_H3K36ME3_N2_L3

Status
Released on 7 July 2010, last updated on 1 May 2014
Organism
Caenorhabditis elegans
Samples (6)
Array (1)
Protocols (8)
Description
modENCODE_submission_175 This submission comes from a modENCODE project of Jason Lieb. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents. We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Keywords: CHIP-chip EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: N2; Developmental Stage: L3 Larva; Genotype: wild type; Sex: mixed Male and Hermaphrodite population; NUMBER OF REPLICATES: 3; EXPERIMENTAL FACTORS: Developmental Stage L3 Larva; temperature 20; Antibody C. elegans AB9050 H3K36ME3 rabbit polyclonal antibody (target is trimethylated Lys-36 of histone H3); Strain N2
Experiment type
ChIP-chip by tiling array 
Contacts
DCC modENCODE <help@modencode.org>, Julie Ahringer, Paulina Kolasinska-Zwierz
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Links