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E-GEOD-22536 - Bile stress response in probiotic Lactobacillus rhamnosus GG

Status
Released on 1 January 2011, last updated on 1 May 2014
Organism
Lactobacillus rhamnosus
Samples (24)
Array (1)
Protocols (7)
Description
Transcriptional profiling of probiotic Lactobacillus rhamnosus strain GG mid-exponential pH-controlled bioreactor cultures before and after exposure to bovine bile (0.2% ox gall). Keywords: bile, stress response Cell samples from four biological replicates were harvested right before (time point 0 min) and 10, 30 and 120 min after bile treatment. Each sample was compared to a common reference sample (time point 0 min, mid-exponential growth phase Lactobacillus rhamnosus GG cultures). A total of 12 hybridizations were performed using balanced dye-swap design. Dyes were balanced between compared sample pairs and between biological replicates.
Experiment type
transcription profiling by array 
Contacts
Kati Laakso <kati.laakso@valio.fi>, Anu Surakka, Dario Greco, Johanna Koponen, Kerttu Koskenniemi, Kirsi Savijoki, Matti Kankainen, Nisse Kalkkinen, Pekka Varmanen, Petri Auvinen, Soile Tynkkynen, Tuomas Salusjärvi, Tuula A Nyman, Willem M de Vos
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-22536.idf.txt
Sample and data relationshipE-GEOD-22536.sdrf.txt
Raw data (1)E-GEOD-22536.raw.1.zip
Processed data (1)E-GEOD-22536.processed.1.zip
Array designA-GEOD-10580.adf.txt
R ExpressionSetE-GEOD-22536.eSet.r
Links