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E-GEOD-22533 - Transcription profiling by array of human breast cancer cell lines treated with fulvestrant

Status
Released on 13 May 2012, last updated on 30 April 2015
Organism
Homo sapiens
Samples (12)
Array (1)
Protocols (8)
Description
A significant fraction of breast cancers exhibit de novo or acquired resistance to estrogen deprivation. To model resistance to aromatase inhibitor (AI) therapy, long-term estrogen-deprived (LTED) derivatives of MCF-7 and HCC-1428 cells were generated through culture for 3 and 7 months under hormone-depleted conditions, respectively. These LTED cells showed sensitivity to the ER downregulator fulvestrant under hormone-depleted conditions, suggesting continued dependence upon ER signaling for hormone-independent growth. To evaluate the role of ER in hormone-independent growth, LTED cells were treated +/- 1 uM fulvestrant x 48 h before RNA was harvested for gene expression analysis. MCF-7/LTED and HCC-1428/LTED cells were treated with 10% DCC-FBS with or without the estrogen receptor antagonist drug fulvestrant for 48 hrs prior to RNA harvest for array analysis. Three replicates per condition.
Experiment type
transcription profiling by array 
Contacts
Todd W Miller <todd.miller@vanderbilt.edu>, Carlos Arteaga, Todd Miller
Citation
ERα-dependent E2F transcription can mediate resistance to estrogen deprivation in human breast cancer. Miller TW, Balko JM, Fox EM, Ghazoui Z, Dunbier A, Anderson H, Dowsett M, Jiang A, Smith RA, Maira SM, Manning HC, González-Angulo AM, Mills GB, Higham C, Chanthaphaychith S, Kuba MG, Miller WR, Shyr Y, Arteaga CL. , PMID:22049316
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-22533.idf.txt
Sample and data relationshipE-GEOD-22533.sdrf.txt
Raw data (1)E-GEOD-22533.raw.1.zip
Processed data (1)E-GEOD-22533.processed.1.zip
Array designA-AFFY-44.adf.txt
Links