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E-GEOD-22528 - Transcription profiling by array of alveolar macrophages from patients with asthma

Status
Released on 29 July 2010, last updated on 1 May 2014
Organism
Homo sapiens
Samples (10)
Array (1)
Protocols (7)
Description
The implication of alveolar macrophages (AM) in asthma, a Th2 disease, has not been well characterized. Thus, the goal of this study is to better characterize AM phenotype of allergic asthmatic compared with normal subjects using genomic expression analyses. Microarray analyses were performed with AM isolated from bronchoalveolar lavage. Robust multiarray analysis (RMA) normalization and Smyth’s moderated t test were used to select differentially expressed genes. Fifty differentially expressed genes were identified. Nineteen have been classified in categories linked to stress or immune responses and among them; nine are part of the heat shock protein (HSP) family. Difference of expression for three (HSPD1, PRNP, SERPINH1) of the five selected genes were validated using real-time reverse transcription–polymerase chain reaction. Enzyme linked immunosorbent assay was used to measure the protein level of heat shock protein 60 (HSP60), the protein encoded by HSPD1, and showed difference in AM protein level between allergic asthmatic and control subjects. In summary, this study suggests that HSP gene family, particularly HSP60, is involved in AM functions in a context of allergic asthma. These results also support the involvement of AM immune functions in the development of an allergic asthmatic response. Ten alveolar macrophage samples from bronchoalveolar lavages have been studied. Five of them are from allergic asthmatic subjects and five from control subjects (without allergy and asthma).
Experiment types
transcription profiling by array, disease state design
Contacts
Catherine Laprise <catherine_laprise@uqac.ca>, Alyse Y Bissonnette, Anne-Marie Madore, Michel Laviolette, Stephanie Perron, Véronique Turmel
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-22528.idf.txt
Sample and data relationshipE-GEOD-22528.sdrf.txt
Raw data (1)E-GEOD-22528.raw.1.zip
Processed data (1)E-GEOD-22528.processed.1.zip
Array designA-AFFY-33.adf.txt
Links