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E-GEOD-22448 - Gene expression data from wild-type and Nlrc5 knockout GM-CSF induced bone marrow dendritic cells infected with Newcastle Disease virus

Status
Released on 22 December 2010, last updated on 27 March 2012
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (8)
Description
Nlrc5 is encoding a Nod-like receptor protein NLRC5/NOD27. To check the involvement of Nlrc5 in antiviral response, we examined gene expression profile in wild-type and Nlrc5 knockout GM-CSF bone marrow macrophage with using microarrays. Bone marrow cells were cultured with GM-CSF and harvested on day 6. The induced dendritic cells were then stimulated with NDV at moi 1, collected at 0, 2, and 4 hours after infection. Total RNA was extracted and subjected to microarray experiment according to manufactures' instructions.
Experiment type
transcription profiling by array 
Contacts
Yutaro Kumagai <ykumagai@biken.osaka-u.ac.jp>, Himanshu Kumar, Shizuo Akira, Taro Kawai
Citation
NLRC5 Deficiency Does Not Influence Cytokine Induction by Virus and Bacteria Infections. Kumar H, Pandey S, Zou J, Kumagai Y, Takahashi K, Akira S, Kawai T. , PMID:21148033
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-22448.idf.txt
Sample and data relationshipE-GEOD-22448.sdrf.txt
Raw data (1)E-GEOD-22448.raw.1.zip
Processed data (1)E-GEOD-22448.processed.1.zip
Array designA-AFFY-45.adf.txt
R ExpressionSetE-GEOD-22448.eSet.r
Links