VALUE = RMA normalized probe intensity
Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was done by the RMA algorithm using GeneSpring 7.2 program. No further adjustments were made to the data in the table.
Affymetrix GeneArray scanner 7G
Lenses were removed from newborn HSF4 null and wildtype mice.
50 ng total RNA was amplified using the NUGEN Ovation™ Biotin RNA Amplification and Labeling System. Amplified cDNA was fragmented and biotin labeled according to manufacturer's instructions.
Total RNA was extracted using RNeasy micro-elute columns (QIAGEN), according to manufacturer's instructions.
Newborn HSF4 null and wildtype newborn mice were used.
2.2 micrograms of amplified/labeled cDNA was hybridized for 17 hr to the Affymetrix array. Genechips® were processed using protocol EukGE_WS2v4 450 for Genechip® fluidics station 450.