Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.
E-GEOD-22269 - Expression data from Saccharomyces cerevisiae treated with Small Molecule Enhancers of Rapamycin (SMERs)
Released on 29 July 2010, last updated on 2 May 2014
The target of rapamycin (TOR) plays a central role in eukaryotic cell growth control. With prevalent hyper-activation of the mTOR pathway in human cancers, novel strategies to enhance TOR pathway inhibition are highly desirable. We used a yeast-based high-throughput chemical genetic screen to identify small-molecule enhancers of rapamycin (SMERs) and used whole genome expression analysis to identify their mechanisms of action. We treated yeast individually with SMERs 1-5, rapamycin, or DMSO (solvent control) for 30 minutes prior to RNA extraction and hybridization on Affymetrix microarrays. Expression profiles of SMER-treated samples were compared to that of DMSO (solvent control) and rapamycin-treated samples to identify gene expression signatures unique to SMER-treated samples.
transcription profiling by array
Mariam Aghajan, Brett Lomenick, Cheng Li, Fei Fu, Ikram Ouni, Jing Huang, Karin Flick, Kendall N Houk, Lan Huang, Manlin Luo, Michael E Jung, Nao Jonai, Nathan Pierce, Ning Zheng, Peter Kaiser, Pierre M del Moral, Rati Verma, Raymond J Deshaies, Robert Damoiseaux, Rui Hao, Xiaobo Tang, Xiaolu Cai, Ying Li