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E-GEOD-22230 - Expression Profile of Chicken Primitive Streak (HH4) Tissues

Status
Released on 29 July 2010, last updated on 27 March 2012
Organism
Gallus gallus
Samples (12)
Array (1)
Protocols (8)
Description
In birds and mammals, all mesoderm cells are generated from the primitive streak. Nascent mesoderm cells contain unique dorso-ventral (D/V) identities depending on their relative ingression position along the streak. Molecular mechanisms controlling this initial phase of mesoderm diversification are not well-understood. Using chick model, we generated high-quality transcriptomic datasets of different streak regions and analyzed their molecular heterogeneity. Streak tissues were dissected from stage-matched HH4 chick embryos and were further divided into four equal pieces along its anterio-posterior length (termed “A”, “B”, “C” and “D”, with “A” representing most anterior and therefore most dorsal and “D” most posterior and therefore most ventral). Each of the four regions was represented by three independent samples (n=3), with each sample containing 5-7 μg total RNA derived from 85-93 pooled pieces. 5 μg of RNA from each sample were used to screen Affymetrix Chicken Genome Array without an amplification step.
Experiment type
transcription profiling by array 
Contacts
Cantas Alev, Guojun Sheng, Hiroki R Ueda, Lars M Jakt, Takeya Kasukawa, Yuping Wu
Citation
Transcriptomic landscape of the primitive streak. Alev C, Wu Y, Kasukawa T, Jakt LM, Ueda HR, Sheng G. , PMID:20667916
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-22230.idf.txt
Sample and data relationshipE-GEOD-22230.sdrf.txt
Raw data (1)E-GEOD-22230.raw.1.zip
Processed data (1)E-GEOD-22230.processed.1.zip
Array designA-AFFY-103.adf.txt
R ExpressionSetE-GEOD-22230.eSet.r
Links