E-GEOD-22222 - Expression profile of Chicken Early Embryonic Endodermal Tissues

Released on 8 June 2013, last updated on 13 May 2014
Gallus gallus
Samples (3)
Array (1)
Protocols (7)
In vertebrates the endoderm which gives rise to the epithelial lining of the digestive tract becomes regionalized along its antero-posterior axis after gastrulation. The molecular basis of the initial step of this regionalization has largely remained unclear. Using chick model, we generated high-quality transcriptomic datasets of different stages/regions of the endoderm and analyzed their molecular heterogeneity. Total RNA from HH stage 4-5 entire definitive endoderm, HH stages 10-11 foregut endoderm and HH stages 8-10 mid/hindgut endoderm were purified. 5 μg of RNA from each sample were used to screen Affymetrix Chicken Genome Array without an amplification step.
Experiment type
transcription profiling by array 
Cantas Alev <geo@ncbi.nlm.nih.gov>, Alev Cantas, Kimiko Fukuda, Martin Jakt, Sadao Yasugi, Sheng Guojun, Wataru Kimura
Investigation descriptionE-GEOD-22222.idf.txt
Sample and data relationshipE-GEOD-22222.sdrf.txt
Raw data (1)E-GEOD-22222.raw.1.zip
Processed data (1)E-GEOD-22222.processed.1.zip
Array designA-AFFY-103.adf.txt