E-GEOD-22222 - Expression profile of Chicken Early Embryonic Endodermal Tissues
Released on 8 June 2013, last updated on 13 May 2014
In vertebrates the endoderm which gives rise to the epithelial lining of the digestive tract becomes regionalized along its antero-posterior axis after gastrulation. The molecular basis of the initial step of this regionalization has largely remained unclear. Using chick model, we generated high-quality transcriptomic datasets of different stages/regions of the endoderm and analyzed their molecular heterogeneity. Total RNA from HH stage 4-5 entire definitive endoderm, HH stages 10-11 foregut endoderm and HH stages 8-10 mid/hindgut endoderm were purified. 5 μg of RNA from each sample were used to screen Affymetrix Chicken Genome Array without an amplification step.
transcription profiling by array
Cantas Alev <firstname.lastname@example.org>, Alev Cantas, Kimiko Fukuda, Martin Jakt, Sadao Yasugi, Sheng Guojun, Wataru Kimura