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E-GEOD-22042 - CAST x C57 QTLs Effect on Gene Expression for Chromosomes 2, 11, and 17

Status
Released on 30 July 2010, last updated on 27 March 2012
Organism
Mus musculus
Samples (72)
Array (1)
Protocols (8)
Description
Three congenic mouse strains were profiled with microarrays and were analyzed using a QTL/Microarray approach to identify candidate genes that regulate biometric phenotypes: HG2D (HG.CAST-(D2Mit329-D2Mit457)), HG11 (HG.CAST-(D11Mit260-D11Mit255, MGI reference: 3771218), and HG17 (HG.CAST-(D17Mit196-D17Mit190); MGI reference: 3771215). All these congenic strains isolate CAST/EiJ (CAST) alleles in a C57BL/6Jhg/hg (C57) background and bare the hg deletion in the high growth locus on chromosome 10. F2 animals from intercrossing congenic males and C57 control females were profiled for gene expresion on brain, liver and gonadal white fat. To detect differential expression produced by allelic effects of biometric QTLs on chromosomes 2, 11, and 17. Three strains were profiled: HG2D, HG11, and HG17, isolating CAST alleles in a high growth (HG) background on chromosomes 2, 11, and 17 respectively. Four biological replicates were assayed per strain for brain, liver and gonadal white adipose tissue. Experiments for each chromosomes were done independently. Only genotype comparisons within tissue in each strain were performed.
Experiment type
transcription profiling by array 
Contacts
Ricardo Alejandro Verdugo <ricardo.a.verdugo@gmail.com>, Craig H Warden, Juan F Medrano, Ricardo A Verdugo
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-22042.idf.txt
Sample and data relationshipE-GEOD-22042.sdrf.txt
Processed data (1)E-GEOD-22042.processed.1.zip
Array designA-MEXP-533.adf.txt
Links