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E-GEOD-21765 - Transcription profiling by array of Arabidopsis gapcp mutants treated with abscisic acid

Status
Released on 28 May 2010, last updated on 1 May 2014
Organism
Arabidopsis thaliana
Samples (6)
Array (1)
Protocols (8)
Description
Glycolytic Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) catalyzes the conversion of glyceraldehyde 3-phospate to 1,3-bisphosphoglycerate by coupling with the reduction of NAD+ to NADH. We generated mutants of the Arabidopsis plastidial GAPDH isoforms (At1g79530, At1g16300; GAPCp1, GAPCp2). gapcp double mutants (gapcp1 gapcp2) display a drastic phenotype of arrested root development and sterility.Complex interactions occurring between ABA and sugar signal transduction pathways have been shown, but the molecular mechanisms connecting both pathways are not well understood. Since we found drastic carbohydrate changes in gapcp1 gapcp2, we studied their response to ABA. by performing a microarray analysis comparing gapcp1 gapcp2 and wild type seedlings after a long term treatment with ABA. 15-day old Arabidopsis seedlings (Col 0) and gapcp1gapcp2 double mutants were used for RNA extraction and hybridization on Affymetrix microarrays.
Experiment types
transcription profiling by array, genetic modification design
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-21765.idf.txt
Sample and data relationshipE-GEOD-21765.sdrf.txt
Raw data (1)E-GEOD-21765.raw.1.zip
Processed data (1)E-GEOD-21765.processed.1.zip
Array designA-AFFY-2.adf.txt
Links