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E-GEOD-21705 - Transcription profiling by array of Arabidopsis mutant for fil, yab3 and yab5 or fil and yab3

Status
Released on 1 August 2010, last updated on 27 January 2012
Organism
Arabidopsis thaliana
Samples (8)
Array (1)
Protocols (7)
Description
In seed plants, leaves are born on radial shoots but unlike shoots they are determinate dorsiventral organs made of flat lamina. YABBY genes are found only in seed plants and in all cases studied, are expressed primarily in lateral organs and in a polar manner. Despite their simple expression, Arabidopsis plants lacking all YABBY gene activities have a wide range of morphological defects in all lateral organs as well as the shoot apical meristem. Here we show that leaves lacking all YABBY activities are initiated as dorsiventral appendages but fail to properly activate lamina programs. In particular, the activation of most CIN-TCPs does not commence, SAM-specific programs are reactivated, and a marginal leaf domain is not established. Altered distribution of auxin signalling and the auxin efflux carrier PIN1, highly reduced venation, initiation of multiple cotyledons, and gradual loss of the SAM accompany these defects. We suggest that YABBY functions were recruited to mould modified shoot systems into flat plant appendages by translating organ polarity into lamina specific programs that include marginal auxin flow and activation a maturation schedule directing determinate growth. Four repeats of wild type 14 DAS plants were used to compare to fil,y3 and fil,y3,y5 mutants of similar age (two repeats of each)
Experiment type
transcription profiling by array 
Contacts
Idan D. Efroni <idane@weizmann.ac.il>, Alexander Goldshmidt, Idan Efroni, John L Bowman, Pia G Sappl, Rajani Sarojam, Sandra K Floyd, Yuval Eshed
Citation
Differentiating Arabidopsis shoots from leaves by combined YABBY activities. Sarojam R, Sappl PG, Goldshmidt A, Efroni I, Floyd SK, Eshed Y, Bowman JL. , PMID:20628155
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-21705.idf.txt
Sample and data relationshipE-GEOD-21705.sdrf.txt
Raw data (1)E-GEOD-21705.raw.1.zip
Processed data (1)E-GEOD-21705.processed.1.zip
Array designA-AFFY-2.adf.txt
R ExpressionSetE-GEOD-21705.eSet.r
Links