Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.
E-GEOD-21652 - Expression data for transcriptional engineering mutants capable of L-tyrosine overproduction
Released on 4 September 2012, last updated on 26 September 2012
We measured transcriptional changes in four strains – P2, rpoD3, rpoA14, and rpoA27 - in an effort to understand mechanisms by which L-tyrosine production is positively influenced by the presence of mutant rpoA- and rpoD-encoded transcriptional components. Strains P2, rpoA14, rpoA27, and rpoD3 were grown in 50 ml MOPS minimal medium to an OD600 of approximately 0.4. Triplicates samples of RNA (on three separate days) were then extracted for hybridization onto Affymetrix microarrays.
transcription profiling by array
Christine Santos <email@example.com>, Christine S Santos, Gregory Stephanopoulos
Rational, combinatorial, and genomic approaches for engineering L-tyrosine production in Escherichia coli. Santos CN, Xiao W, Stephanopoulos G. , PMID:22869698