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E-GEOD-21546 - Gene expression analysis of double positive T cells from different genotypes, unstimulated or stimulated with plate-bound alphaCD3 for 30 min

Status
Released on 8 June 2010, last updated on 10 September 2015
Organism
Mus musculus
Samples (24)
Array (1)
Protocols (8)
Description
Removal of the transcription factor SAP1a member of the Ternary Complex Factor (TCF) group of transcription factors which in conjunction with Serum Response Factor (SRF) has been shown to have a profound effect on positive selection in the thymus. When another TCF Elk1 is knocked out in mice there is no effect on positive selection unless it is on a Sap1a KO background where the phenotype is very severe. We have stimulated isolated double positive T cells (DPs) with anti-CD3 to mimic positive selection and compared basal and stimulated transcription across the four genotypes to discover the downstream targets of Sap1a involved in positive selection. The Affymetrix mouse Chip Mouse430_2 arrays were used to define gene expression profiles in purified DPs with and with out CD3 treatment from 3 mice for each genotype (Wt,SapKO, ElkKO and SAP/Elk double KO)
Experiment type
transcription profiling by array 
Contacts
Citation
Ternary complex factors SAP-1 and Elk-1, but not net, are functionally equivalent in thymocyte development. Cstello P1, Nicoas R, Willoughby J, Wasylyk B, Nordheim A, Treisman R.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-21546.idf.txt
Sample and data relationshipE-GEOD-21546.sdrf.txt
Raw data (2)E-GEOD-21546.raw.1.zip, E-GEOD-21546.raw.2.zip
Processed data (1)E-GEOD-21546.processed.1.zip
Array designA-AFFY-45.adf.txt
Links