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E-GEOD-21360 - Whole-genome microarray analysis of primary, secondary, tertiary and quaternary memory CD8 T cells

Status
Released on 9 July 2010, last updated on 26 June 2012
Organism
Mus musculus
Samples (18)
Array (1)
Protocols (7)
Description
The transcriptome of naive OT-I T cells was compared to memory CD8 T cells after 1, 2, 3, or 4 infection with ovalbumin expressing Listeria monocytogenes (LM-OVA). Naive Thy1.1 OT-I T cells were adoptively transferred into Thy1.2 naive hosts prior to infection with LM-OVA. The resulting memory CD8 T cell population was again adoptively transferred into naive hosts and the recipient mice were again infected with LM-OVA. The adoptive transfer was repeated up to four times to generate memory CD8 T cells with up to four consecutive antigen stimulations. Three individual mice were analyzed for each group. For quaternary memory CD8 T cells, spleens from two to three mice were pooled for each sample. Naive OT-I T cells served as control samples. http://dx.doi.org/10.1016/j.immuni.2010.06.014
Experiment type
transcription profiling by array 
Contacts
Deepa Rai, Hai-Hui Xue, Jaime T Sabel, John T Harty, Thomas C Wirth, Tom Bair, Vladimir P Badovinac
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-21360.idf.txt
Sample and data relationshipE-GEOD-21360.sdrf.txt
Raw data (1)E-GEOD-21360.raw.1.zip
Processed data (1)E-GEOD-21360.processed.1.zip
Array designA-AFFY-130.adf.txt
Links