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E-GEOD-2107 - Hog1 mutant 4 hours exposure to congo red (cell wall chip)

Status
Submitted on 23 December 2004, released on 10 June 2010, last updated on 18 October 2011
Organism
Saccharomyces cerevisiae
Samples (8)
Array (1)
Protocols (2)
Description
S. cerevisiae hog1 mutant strain was grown on YEPD. For Congo Red experiments, yest cells were grown overnight at 24 °C to an optical density 0.8 - 1 (A600). The culture was refreshed to 0.2 O.D and grown at 24 °C for 2h. Next, culture was divided into two parts. One continues growing under same conditions (non-treated culture) while the other was supplemented with Congo Red to a final concentration of 30 µg/ml. Cells were collected at 4hours of growth, frozen at -80 °C and processed for RNA extraction.
Experiment type
unknown experiment type 
Contacts
Javier Arroyo <jarroyo@farm.ucm.es>, Catalina Santiago, Cesar Nombela, Clara Bermejo, Jose M Rodríguez-Peña, Raúl García, Rosa M Pérez-Díaz, Sara Alvarez
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-2107.idf.txt
Sample and data relationshipE-GEOD-2107.sdrf.txt
Processed data (1)E-GEOD-2107.processed.1.zip
Array designA-GEOD-1433.adf.txt
Links