Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.
E-GEOD-2107 - Hog1 mutant 4 hours exposure to congo red (cell wall chip)
Submitted on 23 December 2004, released on 10 June 2010, last updated on 18 October 2011
S. cerevisiae hog1 mutant strain was grown on YEPD. For Congo Red experiments, yest cells were grown overnight at 24 °C to an optical density 0.8 - 1 (A600). The culture was refreshed to 0.2 O.D and grown at 24 °C for 2h. Next, culture was divided into two parts. One continues growing under same conditions (non-treated culture) while the other was supplemented with Congo Red to a final concentration of 30 µg/ml. Cells were collected at 4hours of growth, frozen at -80 °C and processed for RNA extraction.
unknown experiment type
Javier Arroyo <email@example.com>, Catalina Santiago, Cesar Nombela, Clara Bermejo, Jose M Rodríguez-Peña, Raúl García, Rosa M Pérez-Díaz, Sara Alvarez