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E-GEOD-20738 - The shuttling SR-like proteins Gbp2 and Hrb1 mediate the nuclear export of spliced mRNAs

Status
Released on 30 August 2013, last updated on 2 May 2014
Organism
Saccharomyces cerevisiae
Samples (4)
Array (1)
Protocols (8)
Description
Three shuttling SR-like proteins exist in Saccharomyces cerevisiae, Npl3, Gbp2 and Hrb1, that are involved in the nuclear export of mRNAs. In a screen for genes that regulate the export of Gbp2, we identified novel mutants of the splicing factors PRP8 and PRP17 that lead to severe mislocalization defects for Gbp2 and Hrb1, but not Npl3. Microarray and qRT-PCR analyses show that Gbp2 and Hrb1 preferentially bind to transcripts derived from intron-containing genes. Moreover, in contrast to Npl3, Gbp2 and Hrb1 show genetic and physical interactions with late splicing factors such as Prp17 and Prp43. Further, RNA co-immunoprecipitation experiments reveal that, unlike Npl3, association of Gbp2 and Hrb1 with the mRNA requires splicing, and this in turn is required for their Mex67 recruitment. We propose a model in which Gbp2 and Hrb1 are attached to the mRNAs at late stages of splicing to promote the subsequent export of spliced mRNAs. keyword: RIP-chip RNA-IP of endogenously expressed 3-fold C-terminal myc-tagged Gbp2 and Hrb1 in S288C background cells was each performed once in cells grown to a density of 4x10^7 cells/ml and hybridized against a total (input) RNA reference.
Experiment type
transcription profiling by array 
Contacts
Alexandra Hackmann, Birgit Samans, Florian Finkernagel, Haijia Wu, Heike Krebber, Stephanie Heinrich
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-20738.idf.txt
Sample and data relationshipE-GEOD-20738.sdrf.txt
Raw data (1)E-GEOD-20738.raw.1.zip
Processed data (1)E-GEOD-20738.processed.1.zip
Array designA-GEOD-10165.adf.txt
Links