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E-GEOD-20575 - Transcription profiling by array of mouse embryonic and induced pluripotent stem cells

Status
Released on 27 May 2010, last updated on 1 May 2014
Organism
Mus musculus
Samples (4)
Array (1)
Protocols (8)
Description
Pluripotent cells can be derived from somatic cells by either overexpression of defined transcription factors (resulting in induced pluripotent stem cells (iPSCs)) or by nuclear transfer or cloning (resulting in NT-ESCs). To determine whether cloning further reprograms iPSCs, we used iPSCs as donor cells in nuclear transfer experiments. An iPSC clone derived from tail-tip fibroblasts using adenoviral vectors was used as donor cell in nuclear transfer experiments. RNA was isolated from both parental iPSC clone and derivative NT-ESCs lines and analyzed.
Experiment type
transcription profiling by array 
Contacts
Citation
Aberrant silencing of imprinted genes on chromosome 12qF1 in mouse induced pluripotent stem cells. Stadtfeld M, Apostolou E, Akutsu H, Fukuda A, Follett P, Natesan S, Kono T, Shioda T, Hochedlinger K. , PMID:20418860
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-20575.idf.txt
Sample and data relationshipE-GEOD-20575.sdrf.txt
Raw data (1)E-GEOD-20575.raw.1.zip
Processed data (1)E-GEOD-20575.processed.1.zip
Array designA-AFFY-45.adf.txt
R ExpressionSetE-GEOD-20575.eSet.r
Links