Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-20529 - Promoter proximal pausing and its regulation by c-Myc in embryonic stem cells: ChIP-chip

Status
Released on 2 April 2010, last updated on 1 May 2014
Organism
Mus musculus
Samples (16)
Array (1)
Protocols (9)
Description
Recruitment of the RNA Polymerase II (Pol II) transcription initiation apparatus to promoters by specific DNA binding transcription factors is well recognized as a key regulatory step in gene expression. We describe here evidence that promoter-proximal pausing is a general feature of transcription by Pol II in embryonic stem (ES) cells, and thus an additional step where regulation of gene expression may occur. We report here that c-Myc, which occupies a third of actively transcribed genes in ES cells and is a key regulator of cellular proliferation, binds P-TEFb and contributes to release of promoter-proximal paused Pol II at these genes. ChIP-chip data for Pol II and additional factors controlling pause release in mouse ES cells.
Experiment type
ChIP-chip by tiling array 
Contacts
Charles Lin <chazlin@mit.edu>, Amy C Seila, Charles Y Lin, Christopher Burge, Peter B Rahl, Philip A Sharp, Richard A Young, Ryan A Flynn, Scott McCuine
Citation
c-Myc regulates transcriptional pause release. Rahl PB, Lin CY, Seila AC, Flynn RA, McCuine S, Burge CB, Sharp PA, Young RA. , PMID:20434984
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-20529.idf.txt
Sample and data relationshipE-GEOD-20529.sdrf.txt
Raw data (1)E-GEOD-20529.raw.1.zip
Processed data (1)E-GEOD-20529.processed.1.zip
Array designA-GEOD-10099.adf.txt
R ExpressionSetE-GEOD-20529.eSet.r
Links