Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.
E-GEOD-20529 - Promoter proximal pausing and its regulation by c-Myc in embryonic stem cells: ChIP-chip
Released on 2 April 2010, last updated on 1 May 2014
Recruitment of the RNA Polymerase II (Pol II) transcription initiation apparatus to promoters by specific DNA binding transcription factors is well recognized as a key regulatory step in gene expression. We describe here evidence that promoter-proximal pausing is a general feature of transcription by Pol II in embryonic stem (ES) cells, and thus an additional step where regulation of gene expression may occur. We report here that c-Myc, which occupies a third of actively transcribed genes in ES cells and is a key regulator of cellular proliferation, binds P-TEFb and contributes to release of promoter-proximal paused Pol II at these genes. ChIP-chip data for Pol II and additional factors controlling pause release in mouse ES cells.
ChIP-chip by tiling array
Charles Lin <email@example.com>, Amy C Seila, Charles Y Lin, Christopher Burge, Peter B Rahl, Philip A Sharp, Richard A Young, Ryan A Flynn, Scott McCuine