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E-GEOD-2042 - Transcription profiling of mouse embryonic stem cells (ES cells) hree days upon LIF withdrawal (d3) compared to pluripotent cells and of genes whose expression is modulated at d3 under anti-apoptotic conditions (p38 inhibitor PD169316)

Status
Released on 23 November 2007, last updated on 1 May 2014
Organism
Mus musculus
Samples (9)
Arrays (2)
Protocols (5)
Description
Mouse embryonic stem (ES) cells remain pluripotent in vitro when grown in presence of Leukaemia Inhibitory Factor (LIF). LIF starvation leads to apoptosis of some of the ES-derived differentiated cells, together with p38a MAP kinase activation. Apoptosis, but not morphological cell differentiation, is blocked by a p38 inhibitor, PD 169316. To further understand the mechanism of action of this compound, we have identified its specific targets by microarray studies. We report on the global expression profiles of genes expressed at three days upon LIF withdrawal (d3) compared to pluripotent cells and of genes whose expression is modulated at d3 under anti-apoptotic conditions. We showed that at d3 without LIF cells express, earlier than anticipated, specialized cell markers and that when the apoptotic process was impaired, expression of differentiation markers was altered. In addition, functional tests revealed properties of anti-apoptotic proteins not to alter cell pluripotency and a novel role for metallothionein 1 gene which prevents apoptosis of early differentiated cells.
Experiment types
transcription profiling by array, cell type comparison, co-expression, compound treatment
Contact
Citation
Apoptosis and differentiation commitment: novel insights revealed by gene profiling studies in mouse embryonic stem cells. D Duval, M Trouillas, C Thibault, D DembelÈ, F Diemunsch, B Reinhardt, A L Mertz, A Dierich, H Boeuf.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-2042.idf.txt
Sample and data relationshipE-GEOD-2042.sdrf.txt
Raw data (1)E-GEOD-2042.raw.1.zip
Processed data (1)E-GEOD-2042.processed.1.zip
Array designsA-AFFY-3.adf.txt, A-AFFY-6.adf.txt
R ExpressionSetE-GEOD-2042.eSet.r
Links