E-GEOD-20365 - Ethylamine versus succinate cells of Methylobacterium extorquens

Status
Submitted on 17 February 2010, released on 14 May 2010, last updated on 1 May 2014
Organism
Methylobacterium extorquens
Samples (8)
Arrays (2)
Protocols (10)
Description
In order to provide global information on gene expression during growth on C2 compounds, microarray analysis of M. extorquens AM1 cells was carried out, comparing ethylamine-grown cells to succinate-grown cells. This comparison has confirmed previous observations on the inducible nature of some of the enzymes involved in C2 metabolism, such as methylamine (and ethylamine) utilization system (mau), putative enzymes for converting acetaldehyde into acetate and acetyl-CoA, and enzymes of the ethylmalonyl-CoA pathway that has been proposed to operate for assimilation of acetyl-CoA into cell biomass. RNA from ethylamine-grown cells was compared to RNA from succinate-grown cells. Four biological replicates were carried out.
Experiment type
transcription profiling by array 
Contacts
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-20365.idf.txt
Sample and data relationshipE-GEOD-20365.sdrf.txt
Raw data (1)E-GEOD-20365.raw.1.zip
Processed data (1)E-GEOD-20365.processed.1.zip
Array designsA-GEOD-10058.adf.txt, A-GEOD-6262.adf.txt
R ExpressionSetE-GEOD-20365.eSet.r
Links