E-GEOD-20365 - Ethylamine versus succinate cells of Methylobacterium extorquens
Submitted on 17 February 2010, released on 14 May 2010, last updated on 1 May 2014
In order to provide global information on gene expression during growth on C2 compounds, microarray analysis of M. extorquens AM1 cells was carried out, comparing ethylamine-grown cells to succinate-grown cells. This comparison has confirmed previous observations on the inducible nature of some of the enzymes involved in C2 metabolism, such as methylamine (and ethylamine) utilization system (mau), putative enzymes for converting acetaldehyde into acetate and acetyl-CoA, and enzymes of the ethylmalonyl-CoA pathway that has been proposed to operate for assimilation of acetyl-CoA into cell biomass. RNA from ethylamine-grown cells was compared to RNA from succinate-grown cells. Four biological replicates were carried out.
transcription profiling by array
Ludmila Chistoserdova <firstname.lastname@example.org>, Yoko O Miller
Alternative route for glyoxylate consumption during growth on two-carbon compounds by Methylobacterium extorquens AM1. Okubo Y, Yang S, Chistoserdova L, Lidstrom ME.