7 protocols
AccessionType
labeling
Labelled using protocol described in manual "Affymetrix GeneChip Expression Analysis Technical Manual", One-Cycle target labelling
nucleic_acid_extraction
Qiagen RNAeasy kit protocol
feature_extraction
Raw data from the microarrays was normalized at probe-level using MAS5 algorithm. The detection calls (present, marginal, absent) for each probe set was obtained using the GCOS system
hybridization
Biotin Labelled cRNA using "Affymetrix GeneChip Hybridization, Wash and Stain Kit", according to guidelines set out by Affymetrix, Santa Clara, CA
specified_biomaterial_action
The seedlings were treated directly on the agar plates with liquid medium (control) or liquid medium containing 5uM 17-beta-estradiol or 5uM trans-zeatin for 12 and 24 h.
bioassay_data_transformation
ID_REF =
VALUE = MAS5.0-calculated signal intensity
ABS_CALL = the call in an absolute analysis that indicates if the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE = p-value that indicates the significance level of the detection call
image_aquisition
Scanned according to guidelines set out by Affymetrix, Santa Clara, CA.