Labelled using protocol described in manual "Affymetrix GeneChip Expression Analysis Technical Manual", One-Cycle target labelling
Qiagen RNAeasy kit protocol
Raw data from the microarrays was normalized at probe-level using MAS5 algorithm. The detection calls (present, marginal, absent) for each probe set was obtained using the GCOS system
Biotin Labelled cRNA using "Affymetrix GeneChip Hybridization, Wash and Stain Kit", according to guidelines set out by Affymetrix, Santa Clara, CA
The seedlings were treated directly on the agar plates with liquid medium (control) or liquid medium containing 5uM 17-beta-estradiol or 5uM trans-zeatin for 12 and 24 h.
VALUE = MAS5.0-calculated signal intensity
ABS_CALL = the call in an absolute analysis that indicates if the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE = p-value that indicates the significance level of the detection call
Scanned according to guidelines set out by Affymetrix, Santa Clara, CA.