Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.
E-GEOD-20231 - Transcription profiling by array of Arabidopsis primary root tips after treatment with 17-beta-estradiol or trans-zeatin
Released on 28 April 2010, last updated on 1 May 2014
AtIPT8/pga22 seedlings (gain-of-function mutant in Ws background; Sun et al. 2003, Plant Physiology 131, pp167-176) were grown on vertical plates for 7 days in LD. The seedlings were then incubated directly on the plate with medium containing 5 uM 17-beta-estradiol (for induction of the IPT8 gene) or 5 uM trans-zeatin for 12 and 24 h. 5 mm primary root tips were harvested from the seedlings and pooled for microarray analysis. Total RNA was isolated from the samples with the RNeasy Plant Mini Kit from Qiagen. 14 samples were used in this experiment.
transcription profiling by array
Gene regulation by cytokinin in Arabidopsis. Brenner WG, Ramireddy E, Heyl A, Schmülling T. Front Plant Sci 3:8 (2012)