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E-GEOD-20231 - Transcription profiling by array of Arabidopsis primary root tips after treatment with 17-beta-estradiol or trans-zeatin

Released on 28 April 2010, last updated on 1 May 2014
Arabidopsis thaliana
Samples (14)
Array (1)
Protocols (7)
AtIPT8/pga22 seedlings (gain-of-function mutant in Ws background; Sun et al. 2003, Plant Physiology 131, pp167-176) were grown on vertical plates for 7 days in LD. The seedlings were then incubated directly on the plate with medium containing 5 uM 17-beta-estradiol (for induction of the IPT8 gene) or 5 uM trans-zeatin for 12 and 24 h. 5 mm primary root tips were harvested from the seedlings and pooled for microarray analysis. Total RNA was isolated from the samples with the RNeasy Plant Mini Kit from Qiagen. 14 samples were used in this experiment.
Experiment type
transcription profiling by array 
Gene regulation by cytokinin in Arabidopsis. Brenner WG, Ramireddy E, Heyl A, Schmülling T. Front Plant Sci 3:8 (2012)
Investigation descriptionE-GEOD-20231.idf.txt
Sample and data relationshipE-GEOD-20231.sdrf.txt
Raw data (1)
Processed data (1)
Array designA-AFFY-2.adf.txt
R ExpressionSetE-GEOD-20231.eSet.r