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E-GEOD-19984 - Transcription profiling by array of Drosophila mutant for poxn

Status
Released on 17 February 2010, last updated on 30 April 2015
Organism
Drosophila melanogaster
Samples (6)
Array (1)
Protocols (6)
Description
To uncover novel molecules involved in taste detection, we performed a microarray-based screen for genes enriched in taste neurons. Proboscis RNA from flies homozygous for a recessive poxn null mutation was compared to RNA from heterozygous controls. Poxn mutants have a transformation of labellar gustatory chemosensory bristles into mechanosensory bristles and therefore lack most or all taste neurons. Experiment Overall Design: Proboscises of poxn70 homozygous mutant and poxn70 heterozygous mutant males (8-18 days post eclosure) were dissected, and total RNA was harvested in Trizol according to standard trizol protocol. Samples for each microarray were prepared from 164-280 proboscises. We performed 3 biological replicates for each genotype.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
The molecular basis for water taste in Drosophila. Cameron P, Hiroi M, Ngai J, Scott K.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-19984.idf.txt
Sample and data relationshipE-GEOD-19984.sdrf.txt
Raw data (1)E-GEOD-19984.raw.1.zip
Processed data (1)E-GEOD-19984.processed.1.zip
Array designA-AFFY-35.adf.txt
Links