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E-GEOD-19699 - Gene Expression in Human Lung Type II Cells

Status
Released on 30 December 2009, last updated on 2 May 2014
Organism
Homo sapiens
Samples (25)
Array (1)
Protocols (21)
Description
Normal lung function relies on mature function of alveolar type II cels, which have numerous functions including to regulate ion and fluid flux, produce immune molecules, and synthesize and secrete surfactant to stabilize air spaces. Differentiation of type II cells from precursor epithelial cells is accelerated by exposure of cultured cells to glucocorticoid and cAMP. In these studies we used DNA microarray analysis to identify genes of both fetal and adult type II cells that are regulated by glucocorticoid plus cAMP. In the first series of experiments we isolated epithelial cells from fetal human lung and cultured cells for 5 days with or without hormone treatment (dexamethasone plus 8-Br-cAMP plus isobutylmethylxanthine, DCI) and then performed DNA microarray analysis. In the second series of experiments, we isolated type II cells from adult human postmortem lung and cultured cells for 5 days with or without DCI and performed DNA microarray analysis.
Experiment type
transcription profiling by array 
Contacts
Citation
Regulated gene expression in cultured type II cells of adult human lung. Ballard PL, Lee JW, Fang X, Chapin C, Allen L, Segal MR, Fischer H, Illek B, Gonzales LW, Kolla V, Matthay MA. , PMID:20382749
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-19699.idf.txt
Sample and data relationshipE-GEOD-19699.sdrf.txt
Raw data (1)E-GEOD-19699.raw.1.zip
Processed data (1)E-GEOD-19699.processed.1.zip
Array designA-AFFY-33.adf.txt
Links