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E-GEOD-19639 - Transcription profiling by array of human estrogen receptor positive breast cancer cell lines after long-term estrogen deprivation

Status
Released on 23 June 2010, last updated on 30 April 2015
Organism
Homo sapiens
Samples (24)
Array (1)
Protocols (8)
Description
Hyperactivation of phosphatidylinositol-3 kinase (PI3K) promotes escape from hormone dependence in estrogen receptor-positive breast cancer. A significant fraction of breast cancers exhibit de novo or acquired resistance to estrogen deprivation. We used gene expression microarrays to identify genes and pathways that are commonly dysregulated in ER+ cell lines with acquired hormone-independent growth. MCF-7, ZR75-1, MDA-361, and HCC-1428 ER+, estrogen-responsive breast cancer cells were cultured under hormone-depleted conditions (10% DCC-FBS) for several months until sustainable hormone-independent cell populations emerged. Parental and long-term estrogen-deprived (LTED) cells were treated with 10% dextran-coated charcoal-treated fetal bovine serum (DCC-FBS) x 24 hrs prior to RNA harvest for array analysis.
Experiment type
transcription profiling by array 
Contacts
Todd W Miller <todd.miller@vanderbilt.edu>, Carlos Arteaga, Todd Miller
Citation
Hyperactivation of phosphatidylinositol-3 kinase promotes escape from hormone dependence in estrogen receptor-positive human breast cancer. Miller TW, Hennessy BT, González-Angulo AM, Fox EM, Mills GB, Chen H, Higham C, García-Echeverría C, Shyr Y, Arteaga CL.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-19639.idf.txt
Sample and data relationshipE-GEOD-19639.sdrf.txt
Raw data (1)E-GEOD-19639.raw.1.zip
Processed data (1)E-GEOD-19639.processed.1.zip
Array designA-AFFY-44.adf.txt
Links