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E-GEOD-19611 - miRNA profiling in cervical carcinomas, low and high-grade intraepithelial cervical lesions and normal cervical tissues

Status
Released on 1 August 2010, last updated on 1 May 2014
Organism
Homo sapiens
Samples (86)
Array (1)
Protocols (6)
Description
Background. MicroRNAs (miRNAs) are short (~22 nt) non-coding regulatory RNAs that control gene expression at the translational level. Deregulation of miRNA expression has been discovered in a wide variety of tumours and it is now clear that they contribute to cancer development and progression. This prompted the development of miRNA-chips for cancer diagnosis or prognosis, opening a new door to understand carcinogenesis. Cervical cancer is one of the most common cancers in women worldwide. Therefore, there is a strong need for a non-invasive, fast and efficient method to diagnose the disease. We investigated miRNA expression profiles in cervical cancer using a microarray platform developed in house containing probes for mature miRNAs. Results. We have evaluated miRNA expression profiles of a heterogeneous set of cervical tissues from 25 different patients. This set included 19 normal cervical tissues, 4 squamous cell carcinoma, 5 high-grade squamous intraepithelial lesion (HSIL) and 9 low-grade squamous intraepithelial lesion (LSIL) samples. We observed high variability in miRNA expression especially among normal cervical samples, which prevented us from obtaining a unique miRNA expression signature for this tumour type. However, miRNAs deregulation in malignant and pre-malignant cervical tissues was detected after tackling the high variability observed. We were also able to identify putative targets of relevant candidate miRNAs. Conclusions. Our results show that miRNA deregulation may play an important role in the malignant transformation of cervical squamous cells. In addition, deregulated miRNAs highlight new candidate targets allowing a better understanding of the molecular mechanism of this tumour type. In this study we used a common reference design experiment where the common reference used was a commercial RNA from normal cervix (Ambion) and the test samples were 4 pre-treatment squamous cell cervical carcinoma, 7 high-grade Squamous Intraepithelial Lesion (CINII, n=2 and CIN III, n=5) sample, 9 low-grade Squamous Intraepithelial Lesion (CIN I) samples, 19 normal cervix samples and 4 pools of normal cervix samples.
Experiment type
transcription profiling by array 
Contacts
Patrícia Matos Pereira <pereirap@ua.pt>, Ana R Soares, João P Marques, Laura Carreto, Manuel A Santos, Patricia M Pereira
Citation
MicroRNA expression variability in human cervical tissues. Pereira PM, Marques JP, Soares AR, Carreto L, Santos MA. , PMID:20668671
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-19611.idf.txt
Sample and data relationshipE-GEOD-19611.sdrf.txt
Raw data (1)E-GEOD-19611.raw.1.zip
Processed data (1)E-GEOD-19611.processed.1.zip
Array designA-GEOD-7534.adf.txt
Links