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E-GEOD-19497 - Expression data from Fog1+/+ and Fog1 ki/ki mouse megakaryocyte-erythroid progenitors (MEP)

Status
Released on 11 April 2010, last updated on 27 March 2012
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (7)
Description
The transcription co-factor FOG1 interacts with the chromatin remodeling complex NuRD to mediate gene activation and gene repression during hematopoiesis. We have generated mice with a targeted mutation in the endogenous Fog1 locus that results in an N-ternimal mutation in FOG1 that disrupts the interaction with NuRD. We used gene expression microarrays to explore the global transcriptional programs regulated by FOG1 and NuRD in megakaryocyte-erythroid progenitors (MEP) to aid in understanding its role during hematopoiesis. Flow cytometry was used to isolate Megakaryocyte-Erythroid progenitors (MEP) from murine bone marrow. MEP were identified as Lineage neg, kit pos, Sca1 neg, CD34 neg, FcgrII/III low cells.
Experiment type
transcription profiling by array 
Contacts
Gerd A Blobel, Gregory D Gregory, Zhe Zhang
Citation
FOG1 requires NuRD to promote hematopoiesis and maintain lineage fidelity within the megakaryocytic-erythroid compartment. Gregory GD, Miccio A, Bersenev A, Wang Y, Hong W, Zhang Z, Poncz M, Tong W, Blobel GA.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-19497.idf.txt
Sample and data relationshipE-GEOD-19497.sdrf.txt
Raw data (1)E-GEOD-19497.raw.1.zip
Processed data (1)E-GEOD-19497.processed.1.zip
Array designA-AFFY-130.adf.txt
R ExpressionSetE-GEOD-19497.eSet.r
Links