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E-GEOD-19286 - Transcription profiling of mouse aorta genes of APOE-deficient animals receiving the ACE inhibitor captopril

Status
Submitted on 2 December 2009, released on 14 December 2009, last updated on 1 May 2014
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (6)
Description
Microarray gene expression profiling of aorta genes of APOE-deficient mice receiving atherosclerosis treatment with the ACE inhibitor captopril. Hypercholesterolemic APOE-deficient mice were used as a standard model of atherosclerosis to study gene expression changes during atherosclerosis treatment with the ACE inhibitor captopril. Microarray analysis was performed of whole aortas isolated from captopril-treated APOE-deficient mice relative to untreated APOE-deficient mice with overt atherosclerosis, and nontransgenic control mice. Microarray gene expression profiling revealed that captopril-mediated atherosclerosis prevention involved inhibition of aorta-infiltrating immune cells such as pro-atherogenic T lymphocytes and macrophages. Experiment Overall Design: Microarray gene expression profiling was performed of whole aortas isolated from APOE-deficient mice with atherosclerosis relative to captopril-treated APOE-deficient mice, and nontransgenic control mice. Three study groups were analyzed, i.e. 8-months-old untreated APOE-deficient mice with overt atherosclerosis, age-matched APOE-deficient mice treated for 7 months with the angiotensin-converting enzyme (ACE) inhibitor, captopril (20 mg/kg in drinking water), and nontransgenic control C57BL/6J mice. Two biological replicates were made of each group, and total RNA of three aortas was pooled for one gene chip.
Experiment types
transcription profiling by array, unknown experiment type
Contact
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-19286.idf.txt
Sample and data relationshipE-GEOD-19286.sdrf.txt
Raw data (1)E-GEOD-19286.raw.1.zip
Processed data (1)E-GEOD-19286.processed.1.zip
Array designA-AFFY-45.adf.txt
R ExpressionSetE-GEOD-19286.eSet.r
Links