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E-GEOD-18741 - Mucosal responses of healthy humans to three different probiotic Lactobacillus bacteria

Status
Released on 8 September 2010, last updated on 27 March 2012
Organism
Homo sapiens
Samples (28)
Array (1)
Protocols (6)
Description
Probiotic bacteria, specific representatives of bacterial species that are a common part of the human microbiota, are proposed to deliver health benefits to the consumer by modulation of intestinal function via largely unknown molecular mechanisms. To explore in vivo mucosal responses of healthy adults to probiotics, we obtained transcriptomes in an intervention study following a double-blind placebo-controlled cross-over design. In the mucosa of the proximal small intestine of healthy volunteers, probiotic strains from the species Lactobacillus acidophilus, L. casei and L. rhamnosus each induced differential gene regulatory networks and pathways in the human mucosa. Comprehensive analyses revealed that these transcriptional networks regulate major basal mucosal processes, and uncovered remarkable similarity to response profiles obtained for specific bioactive molecules and drugs. This study elucidates how intestinal mucosa of healthy humans perceive different probiotics and provides avenues for rationally designed tests of clinical applications. Keywords: mucosal response of healthy adult humans to lactic acid bacteria This study was set up according to a randomised double-blind cross-over placebo-controlled design. It contains transcriptional profiles from biopsies from 7 healthy individuals after oral intake of three different Lactobacillus species or placebo control. In total, this study includes data from 7 individuals x 4 treatments=28 arrays.
Experiment type
transcription profiling by array 
Contacts
Peter van Baarlen <peter.vanbaarlen@wur.nl>, Cindy van der Meer, Fred J Troost, Guido Hooiveld, Mark Boekschoten, Michiel Kleerebezem, Robert-Jan M Brummer
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-18741.idf.txt
Sample and data relationshipE-GEOD-18741.sdrf.txt
Raw data (1)E-GEOD-18741.raw.1.zip
Processed data (1)E-GEOD-18741.processed.1.zip
Array designA-GEOD-6791.adf.txt
R ExpressionSetE-GEOD-18741.eSet.r
Links