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E-GEOD-18711 - A phylogenetic microarray targeting 16S rRNA genes from the bacterial division Acidobacteria

Status
Released on 24 June 2010, last updated on 10 June 2011
Organism
uncultured Acidobacteria bacterium
Samples (10)
Array (1)
Protocols (7)
Description
Investigation of the phylogenetic diversity of Acidobacteria taxa using PCR amplicons from positive control 16S rRNA templates and total genomic DNA extracted from soil and a soil clay fraction A ten chip study using PCR amplicons from cloned 16S rRNA genes and from diverse soil 16S rRNAs, with PCR primers specific to the Division Acidobacteria. Each chip measures the signal from 42,194 probes (in triplicate) targeting Acidobacteria division, subdivision, and subclades as well as other bacterial phyla. All samples except one (GSM464591) include 2.5 M betaine in the hybridization buffer. Pair files lost due to a computer crash.
Experiment type
other 
Contacts
Mark R Liles <lilesma@auburn.edu>, Balser Teri, Billor Nedret, George Isabelle, Goodman Robert M., Liles Mark, Manske Brian, Rouillard Jean-Marie, Turkmen Ozgur, Zhang Mingzi
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-18711.idf.txt
Sample and data relationshipE-GEOD-18711.sdrf.txt
Processed data (1)E-GEOD-18711.processed.1.zip
Array designA-GEOD-9479.adf.txt
Links