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E-GEOD-18598 - Differentiating 3T3-L1 adipocytes, introduced with siRNA against aof2 and rfk genes, or treated with tranylcypromine

Status
Released on 4 April 2012, last updated on 27 June 2012
Organism
Mus musculus
Samples (4)
Array (1)
Protocols (8)
Description
Adipogenic differentiation and metabolic adaptation are initiated through transcriptional and epigenetic reprogramming. In particular, dynamic changes in histone modifications may play central roles in the rearrangement of gene expression patterns. LSD1 (KDM1) protein, encoded by aof2 gene, is a histone demethylase, which is involved in transcriptional regulation. Since the enzymatic activity of LSD1 is FAD (flavin adenine dinucleotide)-dependent, its effects on gene expression may be influenced by FAD availability. To address the importance of histone demethylation in adipogenic differentiation and function, we performed cDNA microarray in LSD1-deficient 3T3-L1 cells as well as in the cells treated with LSD1 inhibitor tranylcypromine (TC). FAD-synthesizing enyme, riboflavin kinase (RFK) -deficient cells were also subjected to the microarray analysis. 3T3-L1 preadipocytes were transfected with aof2- or rfk- specific siRNA or control siRNA (siGL3) . 24 hours later, cells were subjected to adipogenic induction. 24 hours later, cells were harvested for total RNA extraction. For the TC treatment, TC was added to the adipogenic induction medium.
Experiment type
transcription profiling by array 
Contacts
Citation
FAD-dependent lysine-specific demethylase-1 regulates cellular energy expenditure. Hino S, Sakamoto A, Nagaoka K, Anan K, Wang Y, Mimasu S, Umehara T, Yokoyama S, Kosai K, Nakao M. , PMID:22453831
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-18598.idf.txt
Sample and data relationshipE-GEOD-18598.sdrf.txt
Raw data (1)E-GEOD-18598.raw.1.zip
Processed data (1)E-GEOD-18598.processed.1.zip
Array designA-AFFY-45.adf.txt
R ExpressionSetE-GEOD-18598.eSet.r
Links