E-GEOD-18181 - Meiotic time-course expression profile
Submitted on 20 September 2009, released on 22 May 2010, last updated on 1 May 2014
Saccharomyces cerevisiae SK1
To investigate the dynamics of the meiotic transcriptome and to ensure meiotic synchrony of our samples, we measured RNA abundance in vegetative cells, respiratory (pre-meiotic) cells and in synchronously sporulating cells at various times after transfer to sporulation media (SM). SK1 yeast strain SHy002 (MATa/MATα ho::LYS2/ho::LYS2 leu2::hisG/leu2::hisG lys2/lys2 ura3/ura3)
Meiotic Time Course with samples collected during vegetative growth (YPD), respiratory growth (pre-meiotic/YPA 8 hours) and 0 hr, 1.5hr, 3hr, 4.5hr, 6hr, 9hr and 12hr after transfer to sporulation media (SM).
3 separate biological replicates were used for each condition/time-point.
Total RNA from each sample was hybridized competitively with a genomic DNA common reference.
The expression analysis was carried out from the same biological samples as the FAIRE analysis.
transcription profiling by array
A positive but complex association between meiotic double-strand break hotspots and open chromatin in Saccharomyces cerevisiae. Berchowitz LE, Hanlon SE, Lieb JD, Copenhaver GP.