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E-GEOD-17873 - Investigation of barley brittle stem mutants

Released on 23 July 2010, last updated on 27 March 2012
Hordeum vulgare
Samples (22)
Array (1)
Protocols (7)
The barley brittle stem mutants, fs2, designated X054 and M245, have reduced levels of cellulose compared with their isogenic parents Ohichi and Shiroseto. A custom-designed microarray, based on Agilent technology and including genes involved in cell wall metabolism, was used to compare transcript levels in the mutant and parental lines. For both mutants, the microarray revealed a marked decrease in mRNA for the HvCesA4 cellulose synthase gene in specific zones of stem internodes, and this was confirmed by quantitative PCR. Genes expression was measured for the upper and lower zones from the 4th internodes of stems. Plant were at Zadocks developmental stage 49. Gene expression was compared between mutants and their wildtype parents.
Experiment type
transcription profiling by array 
Ute Baumann <>, Andrew J Harvey, Antony Bacic, Carl R Simmons, Filomena Pettolino, Gang Ma, Geoffrey B Fincher, J A Rafalski, Jillian Taylor, Kanwarpal S Dhugga, Mary Beatty, Neil J Shirley, Rachel A Burton, Scott V Tingey
A customized gene expression microarray reveals that the brittle stem phenotype fs2 of barley is attributable to a retroelement in the HvCesA4 cellulose synthase gene. Burton RA, Ma G, Baumann U, Harvey AJ, Shirley NJ, Taylor J, Pettolino F, Bacic A, Beatty M, Simmons CR, Dhugga KS, Rafalski JA, Tingey SV, Fincher GB. , PMID:20530215
Investigation descriptionE-GEOD-17873.idf.txt
Sample and data relationshipE-GEOD-17873.sdrf.txt
Raw data (1)
Processed data (1)
Array designA-GEOD-9124.adf.txt