E-GEOD-17639 - The human reticulocyte transcriptome (HG-U133_Plus2.0)

Released on 30 November 2009, last updated on 27 March 2012
Homo sapiens
Samples (12)
Array (1)
Protocols (8)
RNA from circulating blood reticulocytes was utilized to provide a robust description of genes transcribed at the final stages of erythroblast maturation. After depletion of leukocytes and platelets, Affymetrix HG-U133Plus 2.0 arrays were hybridized with probe from total RNA isolated from blood sampled from 6 umbilical cords and 6 healthy adult humans. Peripheral blood from 6 normal adults and 6 human umbilical cords were purified to obtain packed red blood cells and their purity was assessed by CELL-DYN4000. The contaminated WBC count was less than 1 out of 1 million cells. Total RNA from purified reticulocytes included in the purified red blood cells was extracted, labeled, and hybridized onto Affymetrix HG-U133Plus 2.0 arrays.
Experiment type
transcription profiling by array 
Jeffery Lynn Miller <jm7f@nih.gov>, Jeffery L Miller, Sung-Ho Goh
Let-7 microRNAs are developmentally regulated in circulating human erythroid cells. Noh SJ, Miller SH, Lee YT, Goh SH, Marincola FM, Stroncek DF, Reed C, Wang E, Miller JL. , Europe PMC 19939273
Investigation descriptionE-GEOD-17639.idf.txt
Sample and data relationshipE-GEOD-17639.sdrf.txt
Raw data (1)E-GEOD-17639.raw.1.zip
Processed data (1)E-GEOD-17639.processed.1.zip
Array designA-AFFY-44.adf.txt
R ExpressionSetE-GEOD-17639.eSet.r