E-GEOD-17639 - The human reticulocyte transcriptome (HG-U133_Plus2.0)
Released on 30 November 2009, last updated on 27 March 2012
RNA from circulating blood reticulocytes was utilized to provide a robust description of genes transcribed at the final stages of erythroblast maturation. After depletion of leukocytes and platelets, Affymetrix HG-U133Plus 2.0 arrays were hybridized with probe from total RNA isolated from blood sampled from 6 umbilical cords and 6 healthy adult humans. Peripheral blood from 6 normal adults and 6 human umbilical cords were purified to obtain packed red blood cells and their purity was assessed by CELL-DYN4000. The contaminated WBC count was less than 1 out of 1 million cells. Total RNA from purified reticulocytes included in the purified red blood cells was extracted, labeled, and hybridized onto Affymetrix HG-U133Plus 2.0 arrays.
transcription profiling by array
Jeffery Lynn Miller <email@example.com>, Jeffery L Miller, Sung-Ho Goh
Let-7 microRNAs are developmentally regulated in circulating human erythroid cells. Noh SJ, Miller SH, Lee YT, Goh SH, Marincola FM, Stroncek DF, Reed C, Wang E, Miller JL. , Europe PMC 19939273