Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.

E-GEOD-17300 - Overexpression of USF1 in HEK293T cells

Status
Released on 14 September 2009, last updated on 27 March 2012
Organism
Homo sapiens
Samples (6)
Array (1)
Protocols (6)
Description
Overexpression of USF1 in HEK293T cells in vitro to ascertain the genes downstream of USF1. Will identify direct targets as well as indirect targets of USF1. Keywords: Overexpression of transcription factor to determine downstream targets. Cloned USF1 from cDNA and transiently transfected into HEK293T cells. Total RNA collected 48 hours after transfection. Empty vector used as control. Expression of USF1 was validated using western blot, and qRT-PCR was used to validate differential expression.
Experiment type
transcription profiling by array 
Contacts
Christopher L Plaisier <plaisier@ucla.edu>, Adriana Huertas-Vazquez, Carlos Aguilar-Salinas, Ivette Cruz-Bautista, Miguel F Herrera, Paivi Pajukanta, Steve Horvath, Teresa Tusie-Luna
Citation
A systems genetics approach implicates USF1, FADS3, and other causal candidate genes for familial combined hyperlipidemia. Plaisier CL, Horvath S, Huertas-Vazquez A, Cruz-Bautista I, Herrera MF, Tusie-Luna T, Aguilar-Salinas C, Pajukanta P. , PMID:19750004
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-17300.idf.txt
Sample and data relationshipE-GEOD-17300.sdrf.txt
Raw data (1)E-GEOD-17300.raw.1.zip
Processed data (1)E-GEOD-17300.processed.1.zip
Array designA-AFFY-44.adf.txt
R ExpressionSetE-GEOD-17300.eSet.r
Links