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E-GEOD-1729 - Transcription profiling by array of human bone marrow samples from acute myeloid leukemia patients

Status
Released on 14 August 2007, last updated on 21 November 2011
Organism
Homo sapiens
Samples (43)
Array (1)
Protocols (2)
Description
Gene expression profile of acute myeloid leukemia. Bone marrow (BM) samples from 43 adult patients with newly de novo diagnosed AML.All samples contained more than 80% blast cells. Total RNA was extracted using Trizol reagent (Life Technologies, Gaithersburg, MD) and purified with RNeasy Mini Kit (Quiagen, Valencia, CA). The RNA integrity was assessed using Agilent 2100 Bioanalyzer (Agilent, Palo Alto, CA). The labeled RNA was then fragmented and hybridazed to HU-133A oligonucleotide array (Affymetrix, Santa Clara, CA), which contained 22,283 probe sets, according to Affymetrix protocols. The arrays were scanned using Gene Array Scanner (Affymetrix).
Experiment types
transcription profiling by array, co-expression, disease state
Contact
Citation
Gene expression profile reveals deregulation of genes with relevant functions in the different subclasses of acute myeloid leukemia. N C Gutierrez, R Lopez-Perez, J M Hernandez, I Isidro, B Gonzalez, M Delgado, E Ferminan, J L Garcia, L Vazquez, M Gonzalez, J F San Miguel.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-1729.idf.txt
Sample and data relationshipE-GEOD-1729.sdrf.txt
Raw data (1)E-GEOD-1729.raw.1.zip
Processed data (1)E-GEOD-1729.processed.1.zip
Array designA-AFFY-33.adf.txt
R ExpressionSetE-GEOD-1729.eSet.r
Links