Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.
E-GEOD-1729 - Transcription profiling by array of human bone marrow samples from acute myeloid leukemia patients
Released on 14 August 2007, last updated on 21 November 2011
Gene expression profile of acute myeloid leukemia. Bone marrow (BM) samples from 43 adult patients with newly de novo diagnosed AML.All samples contained more than 80% blast cells. Total RNA was extracted using Trizol reagent (Life Technologies, Gaithersburg, MD) and purified with RNeasy Mini Kit (Quiagen, Valencia, CA). The RNA integrity was assessed using Agilent 2100 Bioanalyzer (Agilent, Palo Alto, CA). The labeled RNA was then fragmented and hybridazed to HU-133A oligonucleotide array (Affymetrix, Santa Clara, CA), which contained 22,283 probe sets, according to Affymetrix protocols. The arrays were scanned using Gene Array Scanner (Affymetrix).
transcription profiling by array, co-expression, disease state
Gene expression profile reveals deregulation of genes with relevant functions in the different subclasses of acute myeloid leukemia. N C Gutierrez, R Lopez-Perez, J M Hernandez, I Isidro, B Gonzalez, M Delgado, E Ferminan, J L Garcia, L Vazquez, M Gonzalez, J F San Miguel.