5 protocols
AccessionType
array scanning and feature extraction protocol
Title: Affymetrix CEL analysis. Description:
bioassay_data_transformation
Data pre-processed with RMA. Data analysis was performed using the BRB-ARRAYTOOLS software package (available at http://linus.nci.nih.gov/BRB-ArrayTools.html)
labeling
2 µg of total RNA were labeled using the GeneChip® One-Cycle Target Labeling assay kit (Affymetrix), according to the manufacturer´s instructions
nucleic_acid_extraction
extraction with RNeasy Mini Kit (QIAGEN) according to manufacturer´s instructions
specified_biomaterial_action
Ramos cells were transfected in triplicates with pRTS-GFP or pRTS-CA-IKK2, selected with hygromycin and transgene expression was induced with doxycycline (0,5mg/ml) for 48hrs. RNA was isolated with RNeasy mini kit (Qiagen, Venlo, Netherlands)