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E-GEOD-17100 - Gene expression associated with lentiviral expression of PRAME in normal hematopoietic progenitors

Status
Released on 31 October 2009, last updated on 27 March 2012
Organism
Homo sapiens
Samples (12)
Array (1)
Protocols (10)
Description
To identify gene expression that distinguishes hematopoietic cells that express PRAME from those that do not, normal CD34+ cells with forced PRAME expression were compared to cells without PRAME expression in culture over time (days 4, 7, 14) using Affymetrix HU-133A microarrays Normal CD34+ progenitor cells not expressing PRAME were transduced with either PRAME cDNA (no 3' utr) or a corresponding empty vector control by lentiviral vector infection. Two replicates were performed for each condition (PRAME and control) at three experimental time points (days 4, 7, and 14 in culture). A single-stranded linear amplification protocol was used to process total RNA (one microgram) for the microarrays. This protocols is described in detail in PMID 14706461.
Experiment type
transcription profiling by array 
Contacts
Vivian G. Oehler <voehler@u.washington.edu>, Brent L Wood, Carrie L Cummings, Derek L Stirewalt, Era Pogosova-Agadjanyan, Janis L Abkowitz, Jerald P Radich, Katherine A Guthrie, Kathleen Sabo, Mirjam T Epping, Paula Ladne, Taimei Yang, Ted Gooley, Vivian G Oehler, Yaping Shou
Citation
The preferentially expressed antigen in melanoma (PRAME) inhibits myeloid differentiation in normal hematopoietic and leukemic progenitor cells. Oehler VG, Guthrie KA, Cummings CL, Sabo K, Wood BL, Gooley T, Yang T, Epping MT, Shou Y, Pogosova-Agadjanyan E, Ladne P, Stirewalt DL, Abkowitz JL, Radich JP. , PMID:19625708
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-17100.idf.txt
Sample and data relationshipE-GEOD-17100.sdrf.txt
Raw data (1)E-GEOD-17100.raw.1.zip
Processed data (1)E-GEOD-17100.processed.1.zip
Array designA-AFFY-33.adf.txt
R ExpressionSetE-GEOD-17100.eSet.r
Links