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E-GEOD-16674 - Transcription profiling of human K562 cells overexpressing miR-34a

Status
Submitted on 17 June 2009, released on 19 July 2009, last updated on 27 March 2012
Organism
Homo sapiens
Samples (2)
Array (1)
Protocols (5)
Description
miR-34a is strongly induced upon TPA-induced megakaryocyte differentiation of K562 cells. To investigate the gene networks regulated by this miRNA during the process of differentiation we performed gene microarray analysis in K562 cells overexpressing miR-34a or a control sequence. Experiment Overall Design: K562 cells were transfected by nucleofection (Amaxa) with a miR-34a mimic (Dharmacon) or a control sequence with no homology to human genes. 24 hours post-transfection total RNA was extracted using trizol and hybridized on Affimetrix microarrays for comparing gene expression.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Citation
miR-34a contributes to megakaryocytic differentiation of K562 cells independently of p53. Gutman Navarro, Caceres Meire, Bentwich Rigoutsos, Lieberman.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-16674.idf.txt
Sample and data relationshipE-GEOD-16674.sdrf.txt
Raw data (1)E-GEOD-16674.raw.1.zip
Processed data (1)E-GEOD-16674.processed.1.zip
Array designA-AFFY-44.adf.txt
R ExpressionSetE-GEOD-16674.eSet.r
Links