Please note that we have stopped the regular imports of Gene Expression Omnibus (GEO) data into ArrayExpress. This may not be the latest version of this experiment.
E-GEOD-16674 - Transcription profiling of human K562 cells overexpressing miR-34a
Submitted on 17 June 2009, released on 19 July 2009, last updated on 27 March 2012
miR-34a is strongly induced upon TPA-induced megakaryocyte differentiation of K562 cells. To investigate the gene networks regulated by this miRNA during the process of differentiation we performed gene microarray analysis in K562 cells overexpressing miR-34a or a control sequence. Experiment Overall Design: K562 cells were transfected by nucleofection (Amaxa) with a miR-34a mimic (Dharmacon) or a control sequence with no homology to human genes. 24 hours post-transfection total RNA was extracted using trizol and hybridized on Affimetrix microarrays for comparing gene expression.
transcription profiling by array, unknown experiment type
miR-34a contributes to megakaryocytic differentiation of K562 cells independently of p53. Gutman Navarro, Caceres Meire, Bentwich Rigoutsos, Lieberman.