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E-GEOD-16657 - Chromatin Poises miRNA- and Protein-coding Genes for Expression

Status
Released on 10 August 2009, last updated on 1 May 2014
Organism
Homo sapiens
Samples (10)
Protocols (4)
Description
Chromatin modifications have been implicated in regulation of gene expression. While association of certain modifications with expressed or silent genes has been established, it remains unclear how changes in chromatin environment relate to changes in gene expression. In this report, we used ChIP-Seq to analyze the genome-wide changes in chromatin modifications during activation of total human CD4+ T cells by T cell receptor (TCR) signaling. Surprisingly, we found that the chromatin modification patterns at many induced and silenced genes are relatively stable during the short-term activation of resting T cells. Active chromatin modifications were already in place for a majority of inducible protein-coding genes even while the genes were silent in resting cells. Similarly, genes that were silenced upon T cell activation retained positive chromatin modifications even after being silenced. To investigate if these observations are also valid for miRNA-coding genes, we systematically identified promoters for known miRNA genes using epigenetic marks and profiled their expression patterns using deep sequencing. We found that chromatin modifications can poise miRNA-coding genes as well. Our data suggest that miRNA- and protein-coding genes share similar mechanisms of regulation by chromatin modifications, which poise inducible genes for activation in response to environmental stimuli. Keyword(s): Epigenetics Examination of several chromatin modifications in human CD4+ T cells
Experiment type
ChIP-seq 
Contacts
Artem Barski, Keji Zhao, Raja Jothi, Suresh Cuddapah
Citation
Chromatin poises miRNA- and protein-coding genes for expression. Barski A, Jothi R, Cuddapah S, Cui K, Roh TY, Schones DE, Zhao K. , PMID:19713549
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